Norovirus infections (also known as winter vomiting disease), and hepatitis A infections are often caused by people unknowingly eating contaminated bivalve molluscs such as mussels and oysters. This can happen when the bivalve molluscs have been harvested from water polluted with human sewage. Other food types such as soft fruits and salad leaves can also be a source of infection if they are exposed to contaminated water or handled during production and processing by people infected with these viruses. To help food, water and environmental testing laboratories ensure their test procedures are effective, PHE’s Culture Collections, in partnership with the Centre for Environment, Fisheries and Aquaculture Science (Cefas), provide norovirus and hepatitis A reference materials (RMs) in LENTICULE disc format.
These RMs for norovirus (genogroups I and II) and hepatitis A virus can be used as in-run positive extraction controls for quantitative molecular assays such as real time RT-PCR and for method calibration and characterisation. They are also suitable for use in qualitative conventional or semi-quantitative RT-PCR analysis.
The norovirus and hepatitis A RMs in LENTICULE disc format can be used for:
The RMs were developed in parallel with the two part ISO/CEN standard - ISO/TS 15216:2013. Microbiology of food and animal feed - Horizontal method for determination of hepatitis A virus and norovirus in food using real-time RT-PCR which was developed byTAG4 of the European Committee for Standardisation (CEN).
The RMs are supplied with a certificate of analysis which gives reference values. These values are generated using a real time RT-PCR using Cefas’ ISO 17025 accredited procedures for virus quantification based upon ISO/TS 15216-1 (Method for Quantification).
Virus RMs in LENTICULE disc format available:
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