The NCPV catalogue contains many viruses, including numerous under-researched, emerging and re-emerging arboviruses. Several of these have the potential to cause a significant burden of human diseases in the future should events occur that increase human-reservoir/vector interactions such as alterations in climatic conditions.
In Culture Collections, we are able to aid viral research via another one of our collections; the European Collection of Authenticated Cell Cultures (ECACC). ECACC provides a multitude of different authenticated cell lines, some of which can be utilised for the successful culturing of viruses; inclusive of the many specific arboviruses which have the capabilities to cause the next pandemic listed in our article 'From obscurity to notoriety - the evolving nature of viral diseases'.
Below are some selected popular mammalian and mosquito cell lines available from Culture Collections which are suitable for the successful culturing of arboviruses:
• ECACC General Cell Collection: BHK 21 (clone 13): Derived from hamster kidney cells, BHK cell are permissive for many arboviruses. This cell line has a deficient interferon response resulting in more efficient replication and higher titres.
• ECACC General Cell Collection: Vero: Derived from monkey kidney cells, Veros are one of the most frequently used cell lines for culturing arboviruses. Like BHK cells, Veros have a deficient interferon response resulting in more efficient replication and therefore higher titres.
• ECACC General Cell Collection: Vero C1008 [Vero 76, clone E6, Vero E6]: A clonal variant of Vero, E6 cells have noticeable contact inhibition making this cell line suitable for slower growing viruses.
• Aedes aegypti cell line: Aag2-AF319 (19022602): A clonal derivative of the Aag2-AF5 cell line with a Dcr2 knockout mutation. As Dcr2 is a key component of the innate immune response in mosquitoes, this cell line should result in increased viral replication compared to AF5.
• Aedes aegypti cell line: Aag2-AF5 (19022601): A clonal derivative of the Aag-2 cell line from Aedes aegypti, one of the most commonly used mosquito cell lines for culturing arboviruses transmitted by Aedes aegypti mosquitoes.
• Aedes albopictus cell line: C6/36 (89051705): A cell line identified via clonal selection from Aedes albopictus cells. The C6/36 line was noted for susceptibility to many important arboviruses; subsequent studies showed this line has a natural mutation in the Dcr-2 gene explaining why this cell line generated higher titres to compared to other Aedes albopictus cell lines.
To learn more about arboviruses available from the NCPV collection, you can view our arbovirus leaflet.
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