|Supplied by:||European Collection of Authenticated Cell Cultures (ECACC)|
|Culture Type:||Cell line|
|Collection:||ECACC General Collection|
|Cell Line Name:||RK13|
|Citation Guidance:||If use of this culture results in a scientific publication, it should be cited in the publication as: RK13 (ECACC 00021715)|
|Cell Line Description:||
RK13 has been derived from kidney cells of a 5 week old rabbit. The cell line has been shown to be susceptible to pseudorabies virus, rabbitpox virus, myxomatose virus, vaccinia and EAV. Sensitivity to other viruses such as Simian adenovirus and rubella has been reported as well with the production of cytopathic effect (CPE). RK13 has been successfully treated with thiamutin for eradication of mycoplasma.
The Bovine Viral Diarrhoea Virus (BVDV) status of this cell line was previously described as negative. Results of recent multiple molecular tests for BVDV that were undertaken by two independent testing laboratories were equivocal. This means that we cannot confirm the BVDV status of this cell line.
|Tissue of Origin:||Kidney|
|Karyotype:||2n=44, stem-line = 56 (=22%)|
Unless specified otherwise, at the European Collection of Authenticated Cell Cultures (ECACC) we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines. ACDP = Advisory Committee on Dangerous Pathogens (UK)
All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country.
ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.
Hyperlinks to MSDS documents:
Frozen cell cultures Material Safety Data Sheet
Growing cell cultures Material Safety Data Sheet
Nucleic acids derived from cell cultures Material Safety Data Sheet
|Subculture Routine:||Split sub-confluent cultures (70-80%) seeding at 1-3 x10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37°C.; monolayer is confluent after 48-56 hrs and stable for at least 21d; small syncytia in elder cultures are typical.|
|Culture Medium:||MEM(HBSS)+ 2mM Glutamine + 10% Foetal Bovine Serum (FBS).|
|Depositor:||Dr. R. Riebe, Collection of Cell Lines in Veterinary Medicine (CCLV) Federal Research Centre for Virus Diseases of Animals, 17498 Island Riems, Germany|
|References:||Lancet 1963; 2:640|
|Additional Bibliography:||Not specified|
|Patents:||None specified by Depositor|
The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.
Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
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