Any biological material can be Mycoplasma and/or sterility tested if it is cell culture compatible. Cell cultures can be sent either growing or as frozen ampoules.
Please see the following steps on how to submit samples for testing:
If Sending Growing Cultures:
i) Growing cultures should have been passaged twice in the absence of antibiotics. Growing cultures should have been growing for three days since the last medium change. Notification of the time of the last medium change must be made so that the minimum growth time in the absence of antibiotics can be completed if necessary at ECACC.
ii) Growing Cultures should be sent in flasks topped up with medium. The flask cap should be of the non-vented variety and wrapped e.g. in parafilm, to prevent leakage.
If Sending Frozen Cultures:
i) A handling fee will be charged to cover costs of establishing the cell lines in culture.
ii) Details of the cell line growth media and culture conditions must be supplied
Flasks and ampoules should be adequately packed and transported according to UN 602 regulations. Approved UN 602 packaging may be obtained from several suppliers, the details of which are available from any Royal Mail Sales Centre.
If you use a courier service to ship your samples, please contact the company involved for advice on their own approved packaging.
Parcels should be addressed to:
QC Testing Department
Public Health England
Testing is initiated on Tuesdays and Fridays. Notification of dispatch of samples to ECACC is very much appreciated.
Please confirm your country of origin from the list below.