The ECACC PCR detection assay identifies mycoplasma positive samples using primers developed inhouse for screening purposes, and is useful for detecting contamination with the non-cultivable strains of M.hyorhinis.
The PCR method is useful for screening a large number of samples quickly. Results are obtained within a few hours. The presence of contaminant mycoplasma is easily detected using agarose gel electrophoresis by verifying the bands of amplified DNA fragments from test samples against both positive and negative controls.
To ensure the widest range of mycoplasma species can be detected it is recommended that mycoplasma detection by PCR should be carried out in conjunction with mycoplasma detection by Culture Isolation and Indirect Hoechst Stain.
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