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Culture Collections

How to handle frozen cells on receipt

Upon receiving a shipment of frozen cells from ECACC it is important that the end user gives the shipment attention without delay. The technical advice accompanying the cell lines should be consulted before removing ampoules from the dry ice. Correct handling immediately upon receipt is critical to successfully establishing the cell line in the end user’s laboratory.

At the time a cell line is ordered from ECACC, end users should also consider the culture conditions for the new cell line and make sure the appropriate medium will be available when the cells arrive.

Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away. DO NOT use a -80°C freezer as an alternative; this will result in loss of viability.

 

 

 

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